Measuring photoactivation efficiency at the single molecule level

R&D – The ability to determine protein stoichiometry and monitor changes in the balance between monomeric, dimeric and multimeric proteins allows scientists to see the difference between a properly functioning cell and a diseased cell. For this reason, there is a great interest in being able to count proteins and determine their stoichiometry.

Photoactivatable flourescent proteins change their fluorescence property from dark to bright when exposed to light. Through the use of localization-based super-resolution microscopy, researchers are able to photoactivate, image, and follow these genetically encoded fluorescent proteins one at a time to study what is happening inside a cell at the molecular level. more>


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